Metabolic Pathways

Metabolic Pathways

Metabolic Pathways

Impaired interaction of metabolic pathways plays a significant role in disease pathogenesis. Measurement of pathway metabolites and metabolite fluxes completed by mathematical modelling identifies genetic determinants and modifiers such as the microbiome or antibiotics. “Thinking in metabolic networks“ has also become essential for the understanding of monogenic metabolic diseases.

Henning Jessen ORCiD

Faculty of Chemistry and Pharmacy – Institute of Organic Chemistry

RESEARCH: Phosphate Homeostasis, Stringent Response, Phosphorylated Metabolites, Metabolic Messengers, Polyphosphate, Magic Spot Nucleotides, Dinucleoside Polyphosphates, Inositol phosphates and pyrophosphates, Antibiotics

METHODS: Chemical Synthesis, Capillary Electrophoresis and HPLC Mass Spectrometry, Stable Isotope Labeling, Nuclear Magnetic Resonance, Cell Culture

TECHNOLOGIES: CE-MS (Agilent) QQQ, CE-MS (Agilent) QTof, Bruker Avance 400 MHz with cryoprobe

Andreas Bechthold ORCiD

Faculty of Chemistry and Pharmacy – Institute of Pharmaceutical Sciences, Pharmaceutical Biology and Biotechnology

RESEARCH: Metabolism, metabolism signaling, Natural products, antibiotics, biosynthesis of natural products, regulation, cyclic di-GMP, cofactor dependent enzymes, transport of natural products, oxygenases, glycoyltrasnferases, microorganisms in insects

METHODS: Genetic Manipulation of Metabolism, Small Molecule Structure elucidation, Characterization of Natural Products by MS and NMR, Protein structure elucidation, Cryo-EM

TECHNOLOGIES: Triple-Stage-Quadrupol-Massenspektrometer 40-500 + UnliMate 3000 Binäres HPLC-System + DAD (Thermo)

Ute Spiekerkötter ORCiD

Faculty of Medicine and Medical Center – Department of General Pediatrics, Adolescent Medicine and Neonatology

RESEARCH: Inborn errors of metabolism, mitochondrial fatty acid oxidation disorders, risk and severity profiling, pathophysiology and metabolic switches, organ-specific compensation and environmental modifiers, new therapies

METHODS: Molecular and biochemical phenotyping, functional genomics, targeted metabolomics (disease-specific, pathway-specific, multi-pathway), metabolite panels and metabolic labelling, cell and mouse models, clinical studies,  experimental research

TECHNOLOGIES: MetaboCF (DFG listed, RI_00507); Advanced biochemical diagnostic unit: UHPLC Exion/Triple Quadrupole Sciex 5500+ MS, Acquity UPLC/Waters Quattro Premier Triple Quadrupole MS, HPLC Waters 2695 with DAD, Biochrom 30 and 30+ Amino Acid Analyzers, Agilent GC-MS systems, UV-Vis detectors and plate readers

Susana Andrade ORCiD

Faculty of Chemistry and Pharmacy – Institute for Biochemistry

RESEARCH: Metabolite signaling, Metabolite transport, Structure and function of Proteins, Channels, Transporters, Receptors, (Metallo-) Enzymes, Transport mechanisms, Receptor activation, Signal transduction, Protein-protein interaction

METHODS: Protein engineering and isolation, Affinity and size-exclusion chromatography, Molecular Biology, X-ray crystallography, cryo-EM, Proteoliposomes, Lipids, Detergents, Nanoparticles, SSM-/PLB-Electrophysiology, Microscale thermophoresis, Stopped-flow kinetics

TECHNOLOGIES: Planar Lipid Bilayer Electrophysiology (Warner Instr.), Solid-Supported Membrane Electrophysiology (Nanion Tech.), SX-20 Stopped Flow Spectrometer (Applied Photophysics)

Clemens Kreutz ORCiD

Faculty of Medicine and Medical Center – Institute of Medical Biometry and Statistics (IMBI), Methods of Systems Biomedicine (MSB)

RESEARCH: Mathematical Modelling, Metabolic Signaling Networks, Link to Microbiome, Link to Proteome, Methods for Single Cell Analyses

METHODS: Mathematical Models, Benchmarking and Optimal Selection of Computational Methods, Development of Novel Analysis Approaches

TECHNOLOGIES: High Performance Computing

Oliver Einsle ORCiD

Faculty of Chemistry and Pharmacy – Institute of Biochemistry

RESEARCH: Structure and Function of Metabolic Enzymes, , Refactoring of Metabolic Pathways Enzymology, Bioinorganic Chemistry, Structural Biology, Assembly and Biogenesis of Enzymes and Enzyme Complexes

METHODS: Microbiology, Oxic and Anoxic Protein Biochemistry, UV/vis Spectroscopy, EPR Spectroscopy, RT-PCR, Isothermal Titration Calorimetry, Analytical Size-Exclusion Chromatography, Molecular Modelling, Cryo-EM, X-ray Crystallography

TECHNOLOGIES: EPR Sprectrometer (Bruker ElexSys 500), X-ray diffractometer (Rigaku), Cryo-EM (Thermo Fisher)

Luciana Hannibal ORCiD

Faculty of Medicine and Medical Center – Department of General Pediatrics, Adolescent Medicine and Neonatology, MetaboCF Core Facility

RESEARCH: Inborn errors of metabolism, Genetic modifiers of metabolism, Sulfur-metabolism, Redox signalling, Cofactor enzymology and trafficking, Nutrition, Nutritional and Genetic deficiencies of vitamin B12, multiorgan-metabolic integration

METHODS: Metabolic labeling in cell culture, Targeted metabolomic profiling by mass spectrometry, Characterization of pathogenic protein variants, Protein-cofactor interactions, Rapid kinetics by stopped-flow, Biomarker discovery and standardization

TECHNOLOGIES: UHPLC Nexera X2/QTRAP Sciex 6500+ MS, HPLC -TSQ Vantage Triple Quadrupole MS, GC-MS system (7890 GC system coupled to a 5975C mass spectrometer, Agilent), Stopped-Flow with double mixer and dual UV-Vis/Fluorescence detection

Jennifer Andexer ORCiD

Faculty of Chemistry and Pharmacy – Institute of Pharmaceutical Sciences

RESEARCH: Biomimetic Metabolic Pathways, Metabolic Engineering, Multi-enzyme cascades, Biocatalysis, Enzyme Catalysis

METHODS: Genetic Manipulation of Metabolism, Heterologous Expression, Analysis via HPLC and LC-MS

TECHNOLOGIES: HPLC (DAD & ELSD)

Anna Köttgen ORCiD

Faculty of Medicine and Medical Center – Institute of Genetic Epidemiology

RESEARCH: Genetic determinants of Metabolite Levels, Metabolite Transport Proteins, Metabolite handling in Kidney, Inborn Errors of Metabolism, Complex metabolic Diseases, Pharmaco-metabolomics

METHODS: Population-scale genetic screens, whole-exome sequencing, multi-Omics data integration (e.g., metabolomics and genomics), non-targeted metabolomics by MS, statistical data analysis of (non-)targeted MS and NMR metabolomics, pathway analysis