Mitochondria

Mitochondria

Mitochondria

Mitochondria are the central organelle of cellular metabolism. They host key metabolic pathways including the respiratory electron transport chain (ETC) and the Krebs cycle (named after Freiburg scientist Hans A. Krebs). Understanding mitochondria is therefore key to metabolism research. Freiburg Metabolism researchers study the function of ETC complexes, mitochondrial protein import, mitochondrial dynamics and other key aspects of mitochondrial biology. The community is increasingly interested in cell type specific functions of mammalian mitochondrial and their role as signalling hubs.

Bertram Bengsch ORCiD

Faculty of Medicine and Medical Center – Department of Medicine II (Gastroenterology, Hepatology, Endocrinology and Infectious Disease)

RESEARCH: Immunometabolism, Metabolic Signalling, Mitochondria, Single-cell Immune Profiling, Mass Cytometry, Imaging Mass Cytometry, Adaptive Immunity, T cells, T cell exhaustion, Signal Transduction, Inflammatory Diseases, Infectious Diseases, Liver Diseases, Cancer Immunology and Immunotherapy

METHODS: Metabolism-directed flow and mass cytometry, imaging Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Mass cytometry, Imaging Mass cytometry, Hypoxia chambers, Flow cytometry

Luciana Hannibal ORCiD

Faculty of Medicine and Medical Center – Department of General Pediatrics, Adolescent Medicine and Neonatology, MetaboCF Core Facility

RESEARCH: Inborn errors of metabolism, Genetic modifiers of metabolism, Sulfur-metabolism, Redox signalling, Cofactor enzymology and trafficking, Nutrition, Nutritional and Genetic deficiencies of vitamin B12, multiorgan-metabolic integration

METHODS: Metabolic labeling in cell culture, Targeted metabolomic profiling by mass spectrometry, Characterization of pathogenic protein variants, Protein-cofactor interactions, Rapid kinetics by stopped-flow, Biomarker discovery and standardization

TECHNOLOGIES: UHPLC Nexera X2/QTRAP Sciex 6500+ MS, HPLC -TSQ Vantage Triple Quadrupole MS, GC-MS system (7890 GC system coupled to a 5975C mass spectrometer, Agilent), Stopped-Flow with double mixer and dual UV-Vis/Fluorescence detection

Melanie Börries ORCiD

Faculty of Medicine and Medical Center – Institute of Medical Bioinformatics and Systems Medicine

RESEARCH: Multi-Omics data analysis, cancer metabolism, (genetic) analyses of metabolic signaling, identification of disease biomarkers and potential therapeutic options, pipeline development for high-throughput data for clinical research and applications (e.g. Molecular Tumor Board)

METHODS: machine learning methods, development and application of standardized workflows for the analysis, integration and visualization of omics data, single-cell analysis, creating user-friendly databases

TECHNOLOGIES: High-performance computer cluster, 10x Genomics

Nils Wiedemann ORCiD

Faculty of Medicine – Institute of Biochemistry and Molecular Biology

RRSEARCH: Mitochondrial Energy Metabolism, Compartment Specific Metabolomics, Mitochondrial Diseases, Mitochondrial Protein Import and Membrane Insertion, Protein Assembly, Protein Machineries, Cell Biology, Structural Biology

METHODS: Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Untargeted Metabolomics by MS (via collaborations), Subcellular Fractionation, In vitro import of Radiolabelled Proteins into Isolated Mitochondria, Blue-native PAGE, Biochemistry

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Cryomill (Retsch)

Felicitas Bucher ORCiD

Faculty of Medicine and Medical Center – Eye Center

RESEARCH: Signal Transduction, Metabolic Signalling, Mitochondria, Cell Biology, Angiogenesis, Retinal Vasoproliferative Disease

METHODS: Pharmacologic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis (via collaboration), mouse models of retinal vasoproliferative disease (mouse model of oxygen-induced retinopathy, VLDLR Knockout mouse model, Laser-CNV model), RNAseq analysis (outsourced), untargeted Metabolomics (via collaboration)

Dietrich Ruess ORCiD

Faculty of Medicine and Medical Center – Department of General and Visceral Surgery

RESEARCH: Cancer Metabolism, Autophagy, Mitochondria, Pancreatic Cancer, Targeted Therapy, Tumor Microenvironment, Immunooncology

METHODS: Indirect Interference with mutant KRAS driven tumor cell intrinsic metabolic rewiring, Pharmacologic and Genetic Manipulation, Small Molecule Evaluation, Seahorse, Targeted Metabolomics and Metabolic Flux Analysis by MS  (via collaboration)

Daniel Erny ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology, Extracellular Flux Core Facility

RESEARCH: Immunometabolism, Mitochondria, Microglia, CNS Macrophages, Host-Microbiota-Crosstalk, Nervous System Diseases

METHODS: Extracellular Metabolic Flux Analysis, flow cytometry-based metabolic analysis (Oxphos, mitochondrial mass, membrane potential, mitochondrial ROS production,…), high resolution confocal microscopy of mitochondria, transmission electron microscopy, microglia in vitro cultures

Robert Zeiser ORCiD

Faculty of Medicine and Medical Center – Internal Medicine I (Hematology and Oncology)

RESEARCH: Immunometabolism, Cancer Immunotherapy, Allogeneic Stem Cell Transplantation, Graft-versus-Host Disease, Oncogenic Signaling

METHODS: Pharmacologic and Genetic Manipulation of signaling in tumor cells, animal models for acute leukemia and melanoma, Metabolism, Extracellular Metabolic Flux Analysis, bioluminescence imaging

Ian Gentle ORCiD

Faculty of Medicine and Medical Center – Institute for Microbiology and Hygiene

RESEARCH: Immunometabolism, Cancer Metabolism, Mitochondria, Innate Immune Sensing, Macrophages, Signal Transduction, Cell Biology, Cell Death, Inflammation, Crosstalk between cell death signalling and metabolism, Authophagy, metabolic regulation of transcription factors in AML

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Fluorescence microscopy, Extracellular Metabolic Flux Analysis, CRISPR-Cas9

Chris Meisinger ORCiD

Medical Faculty – Institute of Biochemistry and Molecular Biology

RESEARCH: Dynamic Adaptation of the Mitochondrial Proteome upon Stress and Metabolic Switches, Metabolic Signalling, Mitochondria, Signal Transduction, Cell Biology, Nervous System Diseases, Oncogenic Signalling

METHODS: Mitochondrial and Submitochondrial Proteomics and Phosphoproteomics, Global Kinome Profiling for Mitochondrial Signalling Pathways, Import and Assembly of Mitochondrial Proteins, Extracellular Metabolic Flux Analysis

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent)

Heike Rampelt ORCiD

Faculty of Medicine and Medical Center – Institute of Biochemistry and Molecular Biology, ZBMZ

RESEARCH: Mitochondrial Respiration & Physiology, Membrane Architecture, MICOS, F1Fo-ATP Synthase, Respiratory Chain, Membrane Contact Sites, Mitochondrial Biogenesis & Dynamics, Mitochondrial Phospholipids, Biochemistry

METHODS: OXPHOS activity assays, Membrane Potential, Native Complex Assembly / Stability by BN-PAGE, Protein-Protein Interactions, Molecular Biology; Membrane Architecture & Dynamics by EM and Fluorescence Microscopy, Mass Spectrometry (via collaborations)

TECHNOLOGIES: OXPHOS activity assays, Native Complex Assembly / Stability by BN-PAGE, Protein-Protein Interactions

Olaf Groß ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology

RESEARCH: Immunometabolism, Cancer Metabolism, Metabolic Signalling, Mitochondria, Innate Immune Sensing, Macrophage Signal Transduction and Cell Biology, Cell Death, Inflammatory, Infectious, Cardiovascular and Nervous System Diseases

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Small Molecule Screening, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley), Multidrop Reagent Dispenser (Thermo)

Nina Cabezas-Wallscheid ORCiD

Max Planck Institute of Immunobiology and Epigenetics

RESEARCH: Hematopoietic Stem Cell Metabolism, Cancer Stem Cell Metabolism, Metabolic Signalling, Interplay Metabolism-Epigenetics, Dietary Habits and Stem Cell Fate, Quiescence

METHODS: Dietary, Pharmacologic and Genetic Manipulation of Metabolism, Untargeted and Targeted (low input 10,000 cells) Metabolomics by MS, Extracellular Metabolic Flux Analysis

CIBSS, SFB 992, SFB 1425, MSCA-INT: ARCH

Katrin Kierdorf ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology

RESEARCH: Developmental Immunology, Macrophage Development, Macrophage Function, Immunometabolism, Macrophage Metabolism, Metabolic Signalling

METHODS: Ex vivo cell culture platform to study immunometabolism in resident tissue macrophages, tissue normoxic cell cultures, transgenic Drosophila to study conserved immunometabolic signalling

CIBSS, SFB/TRR 167, CRC 1479

Jörg Büscher ORCiD

Max Planck Institute of Immunobiology and Epigenetics, Metabolomics Core Facility

RESEARCH: Metabolomics sample preparation, Metabolomics measurements, Automatic data processing

METHODS: Discovery Metabolomics and Lipidomics, Targeted Metabolomics and Lipidomics, 13C label tracing

TECHNOLOGIES: GC-MS (Agilent), LC-QQQ-MS (Agilent), LC-QTOF-MS (Bruker)

Claudia Jessen-Trefzer ORCiD

Faculty of Chemistry and Pharmacy – Institute of Organic Chemistry

RESEARCH: Infectious Diseases, Mycobacteria, Mycobacterial Metabolite Transporters, Metabolic Signalling, Signal Transduction, Extracellular Nanocompartments, Synthetic Biology, Mycobacterial Membranes, Antibiotics, Drug or Photosensitizer-Metabolite Conjugates and Organometallic Complexes

METHODS: Genetic Manipulation of Metabolism, Small Molecule Screening, Targeted Metabolomics by MS, Protein Isolation and Characterization, Proteomics (in collaboration), Molecular Biology, Microbiology, Organic Synthesis

TECHNOLOGIES: LED array 96-well plate (Teleopt), ChemStudio (Analytics Jena)