Extracellular Flux Analysis

Daniel Erny ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology, Extracellular Flux Core Facility

RESEARCH: Immunometabolism, Mitochondria, Microglia, CNS Macrophages, Host-Microbiota-Crosstalk, Nervous System Diseases

METHODS: Extracellular Metabolic Flux Analysis, flow cytometry-based metabolic analysis (Oxphos, mitochondrial mass, membrane potential, mitochondrial ROS production,…), high resolution confocal microscopy of mitochondria, transmission electron microscopy, microglia in vitro cultures

Felicitas Bucher ORCiD

Faculty of Medicine and Medical Center – Eye Center

RESEARCH: Signal Transduction, Metabolic Signalling, Mitochondria, Cell Biology, Angiogenesis, Retinal Vasoproliferative Disease

METHODS: Pharmacologic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis (via collaboration), mouse models of retinal vasoproliferative disease (mouse model of oxygen-induced retinopathy, VLDLR Knockout mouse model, Laser-CNV model), RNAseq analysis (outsourced), untargeted Metabolomics (via collaboration)

Olaf Groß ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology

RESEARCH: Immunometabolism, Cancer Metabolism, Metabolic Signalling, Mitochondria, Innate Immune Sensing, Macrophage Signal Transduction and Cell Biology, Cell Death, Inflammatory, Infectious, Cardiovascular and Nervous System Diseases

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Small Molecule Screening, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley), Multidrop Reagent Dispenser (Thermo)

Ian Gentle ORCiD

Faculty of Medicine and Medical Center – Institute for Microbiology and Hygiene

RESEARCH: Immunometabolism, Cancer Metabolism, Mitochondria, Innate Immune Sensing, Macrophages, Signal Transduction, Cell Biology, Cell Death, Inflammation, Crosstalk between cell death signalling and metabolism, Authophagy, metabolic regulation of transcription factors in AML

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Fluorescence microscopy, Extracellular Metabolic Flux Analysis, CRISPR-Cas9