Pharmacologic and Genetic Manipulation of Metabolism

RESEARCH: Glucose Metabolism, Endocrine Pancreas Regeneration and Signal Transduction, Stem Cell Biology, Adipose Tissue Biology, Type 1 Diabetes, Type 2 Diabetes, Gestational Diabetes, Obesity, Incretin Hormones, Clinical Trials in Diabetes and Metabolism, Novel Diabetes Medications, Insulin Research, Cardiovascular Disease, Inflammation, NAFLD

METHODS: Cell Biology, Animal Models for Obesity and Diabetes, Pharmacologic and Genetic Manipulation of Metabolism, Clinical Trials in Patients, Endoscopic bariatric interventions

TECHNOLOGIES: Clinical Trial Unit, Phase I-IV, Glucose Clamps in Animals and Humans

RESEARCH: Immunometabolism, Cancer Metabolism, Mitochondria, Innate Immune Sensing, Macrophages, Signal Transduction, Cell Biology, Cell Death, Inflammation, Crosstalk between cell death signalling and metabolism, Authophagy, metabolic regulation of transcription factors in AML

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Fluorescence microscopy, Extracellular Metabolic Flux Analysis, CRISPR-Cas9

RESEARCH: Signaling mechanisms responding to stress and nutrients, ageing and age-related disesases, molecular genetics and biochemistry, C. elegans models of diseases. mTORC1 and mTORC2, SGK-1, signaling mechanisms in tumor biology, programmed cell death, autophagy

METHODS: Genetic dissection of signaling pathways, C. elegans models of human diseases, large scale RNAi screenings, protein interaction studies, DNA and RNA binding, stress biology

TECHNOLOGIES: genome-wide RNAi screenings, mutant screenings, hypoxia chamber, confocal microscopy, C. elegans robotics

RESEARCH: Immunometabolism, Cancer Metabolism, Metabolic Signalling, Mitochondria, Innate Immune Sensing, Macrophage Signal Transduction and Cell Biology, Cell Death, Inflammatory, Infectious, Cardiovascular and Nervous System Diseases

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Small Molecule Screening, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley), Multidrop Reagent Dispenser (Thermo)

RESEARCH: Acquired Metabolic Disease, Metabolic Signalling, Immunometabolism, Adaptive Immunity, T cells, Signal Transduction, Cell Biology, Inflammatory Diseases, Cardiovascular Diseases, Apolipoproteins, Autoimmunity

METHODS: Flow Cytometry, CyTOF, single cell RNA-sequencing, clinical studies, Pharmacologic and genetic manipulation of metabolism, Transcriptomics

TECHNOLOGY: Comprehensive Lab Animal Monitoring System (CLAMS)

RESEARCH: Signal Transduction, Metabolic Signalling, Mitochondria, Cell Biology, Angiogenesis, Retinal Vasoproliferative Disease

METHODS: Pharmacologic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis (via collaboration), mouse models of retinal vasoproliferative disease (mouse model of oxygen-induced retinopathy, VLDLR Knockout mouse model, Laser-CNV model), RNAseq analysis (outsourced), untargeted Metabolomics (via collaboration)

RESEARCH: Immunometabolism, Cancer Immunotherapy, Allogeneic Stem Cell Transplantation, Graft-versus-Host Disease, Oncogenic Signaling

METHODS: Pharmacologic and Genetic Manipulation of signaling in tumor cells, animal models for acute leukemia and melanoma, Metabolism, Extracellular Metabolic Flux Analysis, bioluminescence imaging

RESEARCH: Epigenetic regulation of fatty acids synthesis enzymes, Cancer Metabolism, Regulation of lipids uptake, Hypoxia, Mesenchymal transformation in glioblastoma, Epigenetics, Tumor invasion and migration, Regulation of protein cleavage, Tumor angiogenesis.

METHODS: Pharmacologic and Genetic Manipulation of lipid synthesis enzymes, Gene expression analysis by RNAseq and qPCR, Identification of protein-protein interaction, Mice xenografts

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley)