Immunometabolism

Immunometabolism

Immunometabolism

Specific immune cells and immune responses have distinguishing metabolic characteristics that can be employed to – depending on the disease setting – dampen or enhance immune responses. Freiburg Metabolism researchers study the role of metabolism in specific responses in particular immune cells such as inflammasome activation in macrophages or T cell exhaustion. The community is increasingly interested in modulating immune cell metabolism for cancer therapy.

Claudia Jessen-Trefzer ORCiD

Faculty of Chemistry and Pharmacy – Institute of Organic Chemistry

RESEARCH: Infectious Diseases, Mycobacteria, Mycobacterial Metabolite Transporters, Metabolic Signalling, Signal Transduction, Extracellular Nanocompartments, Synthetic Biology, Mycobacterial Membranes, Antibiotics, Drug or Photosensitizer-Metabolite Conjugates and Organometallic Complexes

METHODS: Genetic Manipulation of Metabolism, Small Molecule Screening, Targeted Metabolomics by MS, Protein Isolation and Characterization, Proteomics (in collaboration), Molecular Biology, Microbiology, Organic Synthesis

TECHNOLOGIES: LED array 96-well plate (Teleopt), ChemStudio (Analytics Jena)

Katrin Kierdorf ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology

RESEARCH: Developmental Immunology, Macrophage Development, Macrophage Function, Immunometabolism, Macrophage Metabolism, Metabolic Signalling

METHODS: Ex vivo cell culture platform to study immunometabolism in resident tissue macrophages, tissue normoxic cell cultures, transgenic Drosophila to study conserved immunometabolic signalling

CIBSS, SFB/TRR 167, CRC 1479

Dennis Wolf ORCiD

Faculty of Medicine and Medical Center – Cardiology and Angiology I, Vascular Immunology Laboratory

RESEARCH: Acquired Metabolic Disease, Metabolic Signalling, Immunometabolism, Adaptive Immunity, T cells, Signal Transduction, Cell Biology, Inflammatory Diseases, Cardiovascular Diseases, Apolipoproteins, Autoimmunity

METHODS: Flow Cytometry, CyTOF, single cell RNA-sequencing, clinical studies, Pharmacologic and genetic manipulation of metabolism, Transcriptomics

TECHNOLOGY: Comprehensive Lab Animal Monitoring System (CLAMS)

Ian Gentle ORCiD

Faculty of Medicine and Medical Center – Institute for Microbiology and Hygiene

RESEARCH: Immunometabolism, Cancer Metabolism, Mitochondria, Innate Immune Sensing, Macrophages, Signal Transduction, Cell Biology, Cell Death, Inflammation, Crosstalk between cell death signalling and metabolism, Authophagy, metabolic regulation of transcription factors in AML

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Fluorescence microscopy, Extracellular Metabolic Flux Analysis, CRISPR-Cas9

Jörg Büscher ORCiD

Max Planck Institute of Immunobiology and Epigenetics, Metabolomics Core Facility

RESEARCH: Metabolomics sample preparation, Metabolomics measurements, Automatic data processing

METHODS: Discovery Metabolomics and Lipidomics, Targeted Metabolomics and Lipidomics, 13C label tracing

TECHNOLOGIES: GC-MS (Agilent), LC-QQQ-MS (Agilent), LC-QTOF-MS (Bruker)

Bertram Bengsch ORCiD

Faculty of Medicine and Medical Center – Department of Medicine II (Gastroenterology, Hepatology, Endocrinology and Infectious Disease)

RESEARCH: Immunometabolism, Metabolic Signalling, Mitochondria, Single-cell Immune Profiling, Mass Cytometry, Imaging Mass Cytometry, Adaptive Immunity, T cells, T cell exhaustion, Signal Transduction, Inflammatory Diseases, Infectious Diseases, Liver Diseases, Cancer Immunology and Immunotherapy

METHODS: Metabolism-directed flow and mass cytometry, imaging Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Mass cytometry, Imaging Mass cytometry, Hypoxia chambers, Flow cytometry

Olaf Groß ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology

RESEARCH: Immunometabolism, Cancer Metabolism, Metabolic Signalling, Mitochondria, Innate Immune Sensing, Macrophage Signal Transduction and Cell Biology, Cell Death, Inflammatory, Infectious, Cardiovascular and Nervous System Diseases

METHODS: Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Small Molecule Screening, Untargeted Metabolomics by MS and NMR (outsourced and via collaborations)

TECHNOLOGIES: Seahorse XFe96 Flux Analyzer (Agilent), Hypoxic Workstations i2 and H35 (Whitley), Multidrop Reagent Dispenser (Thermo)

Melanie Börries ORCiD

Faculty of Medicine and Medical Center – Institute of Medical Bioinformatics and Systems Medicine

RESEARCH: Multi-Omics data analysis, cancer metabolism, (genetic) analyses of metabolic signaling, identification of disease biomarkers and potential therapeutic options, pipeline development for high-throughput data for clinical research and applications (e.g. Molecular Tumor Board)

METHODS: machine learning methods, development and application of standardized workflows for the analysis, integration and visualization of omics data, single-cell analysis, creating user-friendly databases

TECHNOLOGIES: High-performance computer cluster, 10x Genomics

Petya Apostolova ORCiD

Max Planck Institute of Immunobiology and Epigenetics and Medical Center – Department of Hematology, Oncology and Stem Cell Transplantation

RESEARCH: Cancer Metabolism, Immunometabolism, Acute Leukemia, Allogeneic Stem Cell Transplantation, Graft-versus-host disease, Immunotherapy, Immune checkpoint inhibitor-induced autoimmunity

METHODS: Untargeted Metabolomics and Lipidomics by MS, Pharmacologic and Genetic Manipulation of Metabolism, Extracellular Metabolic Flux Analysis, Acute Leukemia and Allogeneic Stem Cell transplantation mouse models

TECHNOLOGIES: Proficiency in the analysis of raw data from LC-MS metabolomics and lipidomics (targeted, untargeted and tracing experiments)

Robert Zeiser ORCiD

Faculty of Medicine and Medical Center – Internal Medicine I (Hematology and Oncology)

RESEARCH: Immunometabolism, Cancer Immunotherapy, Allogeneic Stem Cell Transplantation, Graft-versus-Host Disease, Oncogenic Signaling

METHODS: Pharmacologic and Genetic Manipulation of signaling in tumor cells, animal models for acute leukemia and melanoma, Metabolism, Extracellular Metabolic Flux Analysis, bioluminescence imaging

Nina Cabezas-Wallscheid ORCiD

Max Planck Institute of Immunobiology and Epigenetics

RESEARCH: Hematopoietic Stem Cell Metabolism, Cancer Stem Cell Metabolism, Metabolic Signalling, Interplay Metabolism-Epigenetics, Dietary Habits and Stem Cell Fate, Quiescence

METHODS: Dietary, Pharmacologic and Genetic Manipulation of Metabolism, Untargeted and Targeted (low input 10,000 cells) Metabolomics by MS, Extracellular Metabolic Flux Analysis

CIBSS, SFB 992, SFB 1425, MSCA-INT: ARCH

Daniel Erny ORCiD

Faculty of Medicine and Medical Center – Institute of Neuropathology, Extracellular Flux Core Facility

RESEARCH: Immunometabolism, Mitochondria, Microglia, CNS Macrophages, Host-Microbiota-Crosstalk, Nervous System Diseases

METHODS: Extracellular Metabolic Flux Analysis, flow cytometry-based metabolic analysis (Oxphos, mitochondrial mass, membrane potential, mitochondrial ROS production,…), high resolution confocal microscopy of mitochondria, transmission electron microscopy, microglia in vitro cultures

Dietrich Ruess ORCiD

Faculty of Medicine and Medical Center – Department of General and Visceral Surgery

RESEARCH: Cancer Metabolism, Autophagy, Mitochondria, Pancreatic Cancer, Targeted Therapy, Tumor Microenvironment, Immunooncology

METHODS: Indirect Interference with mutant KRAS driven tumor cell intrinsic metabolic rewiring, Pharmacologic and Genetic Manipulation, Small Molecule Evaluation, Seahorse, Targeted Metabolomics and Metabolic Flux Analysis by MS  (via collaboration)

Stephan Rosshart ORCiD

Faculty of Medicine and Medical Center – Department of Medicine II (Gastroenterology, Hepatology, Endocrinology and Infectious Diseases)

RESEARCH: Immunometabolism (Innate and Adaptive Immunity), Host-Microbiota-Crosstalk via Microbial Metabolites in Inflammatory Diseases, Infectious Diseases and Sepsis, Cancer, Nervous System Diseases, Cardiovascular Diseases, and Kidney Diseases

METHODS: Screening of Bioactive Microbial Molecules (Global Metabolomics, Complex Lipids, SCFA) Outsourced and via Collaborations, Multi-Omics Data Integration (e.g. Metabolomics, CyTOF, Shotgun Metagenomics) In-House and via Collaborations, Animal Models with Natural Microbiota (Wildlings) 

TECHNOLOGIES: BCON BSL-3 BioContainment Rodent Housing System (Plexx), Rodent Surgery Units (Including Microscopic Surgery), Fluorescent-based Cellaca MX High-throughput 24 Sample Automated Cell Counter (Nexcelom Bioscience)